Search results for " Transient transfection"
Article
New Therapies Present Scaling Challenges
One major challenge to a scale-up strategy lies in the crucial step of transient transfection, which is when DNA is introduced into the mammalian cell. The genetic material to be introduced into the m…
Article
Commercial Production of Gene Therapies Using Suspension or Adherent Cell Lines
This is, most often done using transient transfection as a means of integrating the viral genome into the cells, although stable producer cells lines aredeveloped and used (3). Mammalians cells, espec…
Article
A Risk-Based Genetic Characterization Strategy for Recombinant CHO Cell Lines Used for Clinical and Commercial Applications
The authors describe the ways in which manufacturers can mitigate the risks related to the integrity of recombinant transgenes expressed in CHO cells.
By Luhong He, Christopher Frye
Abstract
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Article
Gene Therapies Push Viral Vector Production
Gene Therapies Push Viral Vector Production
Viral vectors show promise as a delivery mechanism for gene therapy, but which virus types are commercially viable?
By Feliza Miraso…
Article
Cell culture process development for AAV vector production in suspension cells
Adaptation of HEK293 suspension cell line for transient transfection and scalable adeno-associated virus (AAV) production.
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Article
Strategizing for Rapid Changeovers in Biologics Manufacturing
Olivier Le Moal/Stock.Adobe.com
By Cynthia A. Challener
Facility and equipment design are important, but the team and its experience matter most.
Through its Operation Warp Speed pro…